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Nonlinear calibration curves are obtained, due to decomposition of the Folin reagent at alkaline pH following addition to the sample that results in incomplete reaction Interferences include agents that acidify the solution, chelate copper, or cause reduction of copper(II) 122 Smith (BCA) Method The Smith total protein assay is also based on the initial complexation of copper (II) with peptides under alkaline conditions, with reduction to copper(I) The ligand BCA is then added in excess, and the purple color (562-nm peak absorbance) develops upon 2:1 binding of BCA with Cu (Fig 12)..

hours of the day or night, if not for your company s European operation, then for its U.S. personnel. As the amount of data grows, the price of storage devices continues to drop dramatically, making it feasible for companies to store all their data. But what happens when the systems crash What happens when disks stop turning What about when your network stops delivering data Does the business stop Must your customers visit your competitor s web site to order their Barbie dolls Should you just send your employees home For how long Can you recover When And how come this stuff never seems to happen to your competitors (Don t worry it does.) The media frenzy surrounding the Y2K nonevent did do some good. The fear of all computers everywhere failing made the average Joe appreciate (even if just a little bit) the direct effect that computers have on his daily life. Although that lesson may have fallen from the society s day-to-day consciousness, at least the impact was there for a short time. As a result, the people who allocate money for the management of computer systems have a little bit more of an appreciation of what can happen when good computers do go bad.

The Y2K problem is certainly not the only issue that has caused problems or at least concerns with computer availability; it just happens to be the best-known example. Some of the others over the last few years include:

the term Vmax may be substituted for k2[E]0; in addition, the constant Km is used to represent (k 1 k2)/k1, so that Eq. 2.16 simpli es to the standard form of the Michaelis Menten Equation (Eq. 2.17): n Vmax S = Km S 2:17

Worldwide terrorism, including the September 11, 2001, attacks on New York and Washington, DC Satellite outages that brought down pagers and other communication devices Attacks by various computer viruses Natural disasters such as floods, tornadoes, and earthquakes The introduction of the euro The emergence of the Internet as a viable social force, comparable to TV or radio as a mass-market influence

Obviously, the actual impact of each of these issues has varied from negligible to serious. But again, each calls attention to the importance and value of computers, and the impact of failures. Downtime on the Internet is like dead air on a television station; it s embarrassing and a mistake that you work hard to prevent.

Note that the Michaelis Menten constant, Km , is related to the dissociation constant for the enzyme substrate complex, Kd ; in fact, Km will always be >Kd , but will approach Kd as k2 approaches 0. It is also noteworthy that when S Km , n Vmax =2; in other words, the reaction rate is half-maximal when the substrate concentration is equal to Km . There are two simpli cations of the Michaelis Menten equation that are of tremendous analytical importance. The rst occurs at low substrate concentration: if S ( Km , then Km S % Km . Under these conditions, Eq. 2.17 simpli es to Eq. 2.18: n Vmax S =Km Constant S 2:18

In this book, we will look at the elements of your computer systems that can fail, whether because of a major event such as the ones just listed, or because of rather mundane problems like the failure of a network router or corruption of a critical file We look at basic system configuration issues, including, but not limited to, physical placement of equipment, logical arrangements of disks, backing up of critical data, and migration of important services from one system to another We will take an end-to-end perspective, because systems are an end-to-end proposition Either everything works or nothing works Rarely is there any middle ground Your users sit in front of their computer screen, trying to complete a task Either the task runs or it doesn t Sometimes it runs, but too slowly.

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