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Contents 5 20 Key High Availability Design Principles #20: Don t Be Cheap #19: Assume Nothing #18: Remove Single Points of Failure (SPOFs) #17: Enforce Security #16: Consolidate Your Servers #15: Watch Your Speed #14: Enforce Change Control #13: Document Everything #12: Employ Service Level Agreements #11: Plan Ahead #10: Test Everything #9: Separate Your Environments #8: Learn from History #7: Design for Growth #6: Choose Mature Software #5: Choose Mature, Reliable Hardware #4: Reuse Configurations #3: Exploit External Resources #2: One Problem, One Solution #1: K.I.S.S. (Keep It Simple . . .) Key Points Backups and Restores The Basic Rules for Backups Do Backups Really Offer High Availability What Should Get Backed Up Back Up the Backups Getting Backups Off-Site Backup Software Commercial or Homegrown Examples of Commercial Backup Software Commercial Backup Software Features Backup Performance Improving Backup Performance: Find the Bottleneck Solving for Performance Backup Styles Incremental Backups Incremental Backups of Databases Shrinking Backup Windows Hot Backups Have Less Data, Save More Time (and Space) Hierarchical Storage Management Archives Synthetic Fulls 75 76 77 78 79 81 82 83 84 87 88 89 90 92 93 94 95 97 98 99 101 104 105 106 108 109 110 110 111 111 113 113 115

Figure 1.1. Nomograph used to estimate total protein and nucleic acid concentrations. [Reprinted, with permission, from Calbiochem, San Diego, CA.]

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Inter-enterprise spaghetti system 434 Inter-enterprise exchange platforms 435 Single Window initiatives 44 Managing business processes 441 Points of departure 442 BPM project opportunity: choosing the processes 443 The top-down approach 444 Expected results 45 Implementing a service architecture 451 Characteristics of an SOA 452 Elements of an SOA infrastructure 453 Applicable norms and standards 51 Brokers 52 Application servers 53 Enterprise Service Bus (ESB) 54 BPM tools ..

1.2. TOTAL PROTEIN The three most common assays for total protein2 are the Lowry (enhanced copper), Smith (bicinchoninic acid, BCA), and Bradford (Coomassie Blue) methods. All are colorimetric methods, and are based on the generation of absorbing species in proportion to the quantity of protein present in the sample. The ninhydrin assay is a recently reported promising method.

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Use More Hardware Host-Free Backups Third-Mirror Breakoff Sophisticated Software Features Copy-on-Write Snapshots Multiplexed Backups Fast and Flash Backup 135 135 136 138 138 140 141

121 Lowry Method The Lowry, or enhanced alkaline copper method, begins with the addition of an alkaline solution of Cu2 to the sample The copper forms a complex with nitrogen atoms in the peptide bonds of proteins under these conditions, and is reduced to Cu The Cu , along with the R groups of tyrosine, tryptophan, and cysteine residues of the protein then react with the added Folin Ciocalteau reagent, which contains sodium tungstate, sodium molybdate, phosphoric acid, and HCl (W6 /Mo6 ) During this reaction, the Cu is oxidized to Cu3 , which then reacts with the pep CO Co tide backbone to produce imino peptide (R1 N C(R2) R3) plus Cu2 , and the Folin reagent is reduced to become molybdenum tungsten blue Absorbance is measured in glass or polystyrene cuvettes at 720 nm, or if this value is too high (>2), at 500 nm.

Handling Backup Tapes and Data General Backup Security Restores Disk Space Requirements for Restores Summary Key Points 7 Highly Available Data Management Four Fundamental Truths Likelihood of Failure of Disks Data on Disks Protecting Data Ensuring Data Accessibility Six Independent Layers of Data Storage and Management Disk Hardware and Connectivity Terminology SCSI Fibre Channel Multipathing Multihosting Disk Array Hot Swapping Logical Units (LUNs) and Volumes JBOD (Just a Bunch of Disks) Hot Spares Write Cache Storage Area Network (SAN) RAID Technology RAID Levels RAID-0: Striping RAID-1: Mirroring Combining RAID-0 and RAID-1 RAID-2: Hamming Encoding RAID-3, -4, and -5: Parity RAID Other RAID Variants Hardware RAID Disk Arrays Software RAID Logical Volume Management Disk Space and Filesystems Large Disks or Small Disks What Happens When a LUN Fills Up

A calibration curve obtained with standard protein solutions [eg, bovine serum albumin (BSA)] is used to obtain total protein in the unknown Under optimum conditions, and in the absence of reactive side chains, it has been shown that two electrons are transferred per tetrapeptide unit; however, proteins, with signi cant proline or hydroxyproline content, or with side chains that can complex copper (such as glutamate) yield less color The side chains of cysteine, tyrosine and tryptophan contribute one, four and four electrons, respectively3 Note that different proteins will produce different color intensities, primarily as a result of different tyrosine and tryptophan contents With reagents prepared in advance, the Lowry assay requires $ 1 h A 400-mL sample is required, containing 2 100-mg protein (5 250 mg/mL).

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