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To understand how enzymes are used in bioanalytical methods, and how their concentrations are represented and determined, it is rst necessary to examine the kinetics of one- and two-substrate enzymatic reactions. 2.5. ENZYME KINETICS The dramatic increases in reaction rates that occur in enzyme-catalyzed reactions can be seen for representative systems in the data given in Table 2.2.4 The hydrolysis of the representative amide benzamide by acid or base yields second-order rate constants that are over six orders of magnitude lower than that measured for benzoyl-L-tyrosinamide in the presence of the enzyme a-chymotrypsin. An even more dramatic rate enhancement is observed for the hydrolysis of urea: The acidcatalyzed hydrolysis is nearly 13 orders of magnitude slower than hydrolysis with the enzyme urease. The disprotionation of hydrogen peroxide into water and molecular oxygen is enhanced by a factor of $1 million in the presence of catalase. Enzymes derive both their selectivities and their reaction rate enhancements by the formation of enzyme substrate complexes. This complex formation results in a transition state for the reaction, that lowers Gz , the activation energy, but does not affect the net free energy for the conversion of substrate to product. This nding is represented in Figure 2.5, for the simple one-substrate enzyme reaction shown in Eq. 2.10: E S $ E S !E P 2:10

There is no requirement for the disks to be a certain type or brand; the software replicates from its own logical volumes (as described in 7) to another system and its set of logical volumes. There is no requirement for a specialized network; a standard TCP/IP network is usually just fine. If you add software-based replication to an existing WAN, it is likely, however, that your network will see a rather substantial decline in performance and throughput due to the additional data traffic. Because of its open nature, software-based replication tends to be a less expensive solution than hardware-based solutions, especially when considered from end to end and including disk and network hardware. Surprisingly, benchmarks reveal that software-based replication tends to have far less impact on systems and applications than hardware solutions do. (More on this in Is Software Replication Really Faster .) There are no specialized hardware requirements for repeaters, protocol converters, or wide area networking hardware. Because of the way that hardware-based replication copies its data, generally you ll usually need to keep more copies of the data on separate sets of disks than you will with software-based replication, saving you money on hardware.

One disadvantage to software-based RAID is that you can only replicate data between systems that support the software that does the replication. With hardware-based RAID, you could replicate data from your Amiga to a Palm OS PDA, as long as you can figure out how to connect those to the disk arrays.

where E is the enzyme, S is the substrate, E S is the enzyme substrate complex, and P is the product of the reaction. The critical rst step in enzyme-catalyzed reactions is the formation of E S, and this is usually represented as a simple association reaction, as in Eq. 2.10. Because it is the E S complex that is the reactant in the substrate conversion step, its concentration determines the rate of the reaction; it follows, then, that the reaction rate

In our experience, and counterintuitively, software-based replication is actually faster than hardware-based replication. The following are some of the reasons why:

Software replication solutions generally replicate smaller blocks of data than hardware-based solutions. Less data means that the transactions finish faster, and with less network traffic being generated. Smaller

The activities can be manual or automatic (i.e., performed by computing applications). Figure 3.33 provides a simplified representation of the structure of a business process. In 2004, BPMI.org (Business Process Management Initiative) published version 1 of the specifications for the BPMN (Business Process Management Notation), a graphical notation for describing business processes in the form of diagrams (BPD Business Process Diagram) [BPM 04]. In June 2005, BPMI.org and OMG (Object Management Group ) merged their activities around BPM in the group BMI DTF (Business Modeling and Integration Domain Task Force) within the OMG. The adoption of BPMN as an OMG standard is being finalized at the time of writing. The BPMN notation is projected for extension, if necessary, to allow the addition of non-standard elements or of artifacts for satisfying particular needs, for example, to respond to requirements linked to a particular vertical sector. BPMN does not prejudge the language that will be used for the execution of the business processes, but offers an example of correspondence with the Business Process Execution Language (BPEL see section 3.3.2). This notation is composed of four categories of basic elements: connections (connecting objects); actions (flow objects); groups of elements (swimlanes); artifacts.

TABLE 2.2. Examples of the Catalytic Power of Enzymesa Substrate Amide (hydrolysis) -Benzamide -Benzamide -Benzoyl-L-tyrosinamide Urea (hydrolysis) Hydrogen peroxide

18

chunks also mean that less unchanged data is replicated. A small local write can generate a much larger replicated write via hardware-based replication, which usually replicates data in the same-sized chunks each time. The same local write on most software-based replication products will send less data while replicating.