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Figure 10.2. Calibration curve for hemagglutinin molecular weight determination. [Reprinted, by permission, from D. J. Holme and H. Peck, Analytical Biochemistry , Longman, New York, 1998. # Addison Wesley Longman Limited 1998.]

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Hardware-based replication adds its overhead when it performs the write; that s when it begins the process of spawning off the replicated copy of the data. The moment of the actual disk write is the slowest part of the transaction. By spawning the write at that point during the process, the slowest part of the transaction is made even slower. Software-based replication is done much earlier in the process so that more of the writes wind up being done in parallel, making better use of resources. While it s certainly true that hardware-based replication s work is done on an independent CPU located inside the disk array, it can still have a significant effect on the computer system s CPU. So that it does not get swamped by incoming replication requests, some hardware solutions will put the CPU into an uninterruptible disk-wait state while the transaction is sent across the network and the confirmation is making the return trip. During a disk-wait, no other activity can be performed on the host CPU. On a busy system with a lot of data being replicated, this will seriously slow down the host as its CPU essentially stops working for the duration of the replication. Some hardware-replication solutions do not use TCP/IP as their native network. Unless your enterprise uses one of these other networks (such as ESCON), it may be necessary to slow down the delivery of each data packet across the network by encapsulating it inside a TCP/IP packet and then unencapsulating it on the other end. The encapsulation also serves to make the packet larger, further slowing down the replication process.

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We certainly understand that product improvements are being made all the time and that what is true about performance as we write this material may or may not be true when you read it. Therefore, we once again urge our readers to carefully evaluate the performance of any replication solution that you may be considering, and that you run relevant benchmarks to determine which solution will balance the best data protection with the least impact on application and system performance.

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in measuring very small migration distances. The molecular weight of hemagglutinin was thus determined to be 160 kDa, which is within the linear region of the calibration curve. If the unknown protein showed a molecular weight in excess of $ 300 kDa, the experiment would be repeated using a lower range of %T values and a series of standard proteins of higher molecular weight. This technique yields molecular weight values that are accurate to within 5%. 10.3. DETERMINATION OF PROTEIN SUBUNIT COMPOSITION AND SUBUNIT MOLECULAR WEIGHTS2 Tertiary and quaternary structure in multisubunit proteins is maintained in part by the presence of disul de bonds between cysteine residues. When such a protein is exposed to a reducing agent such as mercaptoethanol or dithiothreitol, the disul de bonds are converted to sulfhydryl groups, the subunits dissociate and the tertiary structure of the subunits is disrupted. In the presence of SDS, such protein subunits yield uniformly negative species. Proteins will bind SDS in a 1.4 g SDS to 1-g protein ratio, effectively obliterating any native charge on the protein, and this uniform negative charge/mass ratio results in electrophoretic migration from cathode to anode with distances that depend only on the size of the polypeptide chain. The SDS polyacrylamide gel results in a simple molecular sieving action that allows small proteins to migrate rapidly, and restricts the migration of large proteins. Plots of log(relative molecular mass) (RMM, molecular weight) against relative mobility (migration distance) are linear, and allow the determination of unknown molecular weights. Figure 10.3 and Table 10.2 show data obtained for seven

When you boil it down to its base components, filesystem-based replication is much like tape backup: a point-in-time copy of the files in a filesystem.

3.3.1.1. Connections These are the graphical representations used to connect elements to each other. There are three types of connection: execution sequence (sequence flow); exchange of messages (message flow); associations. Table 3.1 below provides a brief description of these elements.

Protein Cytochrome c (muscle) Myoglobin (equine skeletal muscle) IgG Light chain Carbonic anhydrase (bovine) Ovalbumin Albumin (human) Transferrin (human)

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