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In some failover configurations, the takeover server will boot up with no network connectivity, apart from the heartbeat connections to its partner server. The side effects of this lack of public network connectivity at boot time can be seriously detrimental to the server. Naming services will fail, as will email, network printing, and any other network services. If a public network interface is not present, the initial implementation of the FMS will be greatly complicated because those services will need to be disabled at boot time, only coming to life when the server receives a failover. It is best to configure failover servers with an additional interface connected to the public network. We call this network connection the administrative interface because it is used solely for administrative purposes. Besides providing basic network connectivity and services, this connection allows system administrators a guaranteed connection to a particular server rather than to an anonymous server providing a particular service. The administrative interface allows an administrator to log in to a failed server and investigate the cause of a failover before the server is put back into public use. This interface should not be publicized to users, as it is not highly available and does not provide them a guaranteed route to their applications. Just because you think that your administrative and heartbeat networks are private does not mean that they are. Clever and misconfigured dynamic routers have been known to find and use networks that they should not. Make sure that the PRIVATE flag is set on any network routes that should not have public traffic flowing over it.

There are two kinds of disks in any failover configuration. There are the private disks that contain each host s private system information. These disks are dedicated to one, and only one, server, so they should be electrically independent from the shared disks, which can be accessed by both servers in the pair. The shared disks contain the critical data that is needed by the applications for which the servers were made highly available in the first place. To maximize system availability, all disks must have a level of redundancy, as discussed in 7, Highly Available Data Management. Ideally, all disks should be mirrored from one disk controller to another, and from one disk cabinet to another. If the system is important enough to cluster, then the system is important enough to have its disks mirrored.

Moreover, by comparison of homologous enzymes from thermophilic and nonthermophilic microorganisms, it is known that introduction of disul de bridges as well as increased numbers of proline residues increase protein stability Rational design methods have been applied to a glucose dehydrogenase enzyme to improve thermal stability, EDTA tolerance, and substrate selectivity1 Pyrroloquinoline quinone (PQQ) enzymes are found in Gram-negative bacteria This family of enzymes uses PQQ as the prosthetic group PQQ glucose dehydrogenase (PQQGDH) is a monomeric membrane-bound protein with a MW of 87 kDa An improved version of this enzyme would have an important application in glucose sensors; the enzyme commonly used in these devices, GO, cannot be used under anaerobic conditions unless an oxidant is present to perform the function of O2 Yoshida et al1 used homologous recombination of PQQGDH genes from Escherichia coli and Acinetobacter calcoaceticus, and previous knowledge to generate several chimera proteins.

Private disks contain the operating system, the system identity, swap space, and the FMS executables. In order to start the clustering software at boot time,

15

Their goals were to improve the PQQ binding constant (assayed as EDTA tolerance since a divalent ions is required for holoenzyme formation), to increase temperature stability, and to improve substrate selectivity Their procedure is shown schematically in Figure 81 Seven genetic variants were produced from the original E coli gene Site-directed mutagenesis was used to substitute the His residue in position 775 to Asn (His775Asn) This mutation improves the substrate speci city but simultaneously causes a decrease in thermal stability Some regions of A calcoaceticus that are responsible for EDTA resistance and for thermal stability were inserted into the E coli gene by homologous recombination The chimeric genes obtained were used to transform E coli cells, with each single clone expressing one variant of PQQ glucose dehydrogenase The enzyme was isolated and assayed in a buffer containing PQQ to form the holoenzyme, and a colorimetric indicator.

3.1.3.3. Thin and thick adapters With respect to integration solutions, we can traditionally distinguish two categories of adapters: light or technical adapters ( thin adapters ) and business adapters ( thick adapters ): Light (Figure 3.10) or technical adapters are characterized by the fact that they do not require the installation of any component on the platform which hosts the application, because they use the communication functions already present on that platform. They therefore accomplish only communication between the application to be integrated and the core of the integration solution, and ensure the transport of information in the proprietary format for each application. All transformation and routing processes are performed on the platform which hosts the core of the integration solution. Below, we provide some advantages and drawbacks for this type of adapter.