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marketing with claims of larger limits, it was nearly impossible to implement larger clusters With the advent of SANs, cluster sizes have grown immensely Today s products support clusters of 32 and 64 nodes, with larger clusters likely, especially as blade computers become more prominent (for more on blade computing, see 22, A Brief Look Ahead ) Although cluster configurations can grow quite large, some shops have been reluctant to grow clusters beyond a certain size The largest cluster in production that is running on commercial FMS that we are aware of is a 20-node cluster running VERITAS Cluster Server on Solaris Most clusters tend to stay smaller than that; with 7 to 10 nodes being the most common size after 2-node clusters (which are easily the most popular size) When clusters get larger than that, configuration files get large, and the clusters can become harder to manage.

Figure 8.5. In vivo methods allow connection between phenotype and genotype using cellular machinery. Synthesized proteins remain in the cytoplasm (or are secreted) in the nondisplay method. Cell surface display is a more recently introduced technique that uses special plasmids in order to produce proteins capable of transport into and retention by the cell membrane.

Plus, in the unlikely event that the FMS itself causes an outage, the effects become much more significant when that likelihood is multiplied across many servers In terms of the technology covered in this chapter, very little actually changes when you discuss larger clusters Shared disks are still required, albeit SAN-based disks Each host still needs its three kinds of networks And, of course, critical applications are required; without them, there s little point to clustering at all Probably the biggest change due to the increase in cluster size is in the heartbeat networks When cluster sizes exceed two nodes, it becomes necessary to use hubs to build the heartbeat networks When you use hubs to manage your heartbeat networks, make sure that the hubs do not add latency to the traffic, and merely forward packets on to their appropriate destination.

- Need to deploy components on the application platforms with frequent updates of the main parameter settings - Legacy applications in the enterprise not taken into account by these adapters - High price of these adapters since they may incorporate advanced transformation functions As so often, when we compare different possibilities, there is no absolute truth In most cases, the right solution consists of finding a balance between the use of thin adapters and thick adapters 3134 Tools for developing adapters Since it is unlikely that the standard solutions on the market will supply the complete set of adapters required to integrate all the types of applications that can be found in different information systems, it is strongly recommended to acquire a tool set for developing the missing adapters.

Each heartbeat hub should be on a separate power source Larger clusters also enable many more complex and economical failover configurations besides the traditional active-passive and active-active We will examine cluster configurations in detail in 17..

8.4.2. Phage Display (in vivo) Phage display begins with the ligation of each member of the genetic library to a section of viral DNA that codes for one of the coat proteins of the virus. The most commonly used virus is the lamentous M13 phage, a common lab strain, and the coat protein is the gene III protein (gIIIp). The population of viruses is used to infect a bacterial culture; the resultant daughter phages display the evolved proteins and contain the genetic code responsible for their displayed phenotypic variants. A variation of this method involves the construction of a plasmid containing the evolved variant DNA linked to gIIIp DNA. The plasmid is introduced to E. coli, which are cultured before M13 phage is added to the culture. This infection is necessary to allow the production of all the phage proteins, and therefore the production of phages in the bacterial cell occurs at the expense of the host cellular machinery. When phages are assembled in the bacterial cell, the viral capsids display the evolved proteins on their surfaces and at the same time contain the corresponding gene, as shown in Figure 8.6. Phage display is often used when molecular af nity molecules (e.g., antibodies) are being selected. An excellent description of this display system has been written by Johnsson and Ge.15

To maximize application availability, you need a second system that can take over the application if the first system fails. Be sure your application can actually run on the takeover server. Think of your computer as a service-delivery mechanism. Your applications need not run on any one particular system, but rather on any of the systems in a cluster. Plan out your IP address space and host-naming conventions carefully. You re going to use quite a few of them, and you want to be sure you can easily identify hosts and host-network connections.

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1) Fused genes are used to transform bacterial cells 2) Phage M13 infect transformed cells mRNA 3) Proteins and DNA are synthesized