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2:7 In addition to the E.C. number, the source of a particular enzyme is usually given, listing the species and the organ or tissue from which it was isolated. 2.4. ENZYMES IN BIOANALYTICAL CHEMISTRY Enzymes can be employed to measure substrate concentrations as well as the concentrations of species that affect the catalytic activity of the enzyme toward its substrate, such as activators and inhibitors. The rst known enzymatic assay was reported by Osann in 1845: hydrogen peroxide (H2O2) was quantitated using the enzyme peroxidase. In 1851, Schonbein reported a detection limit of 1 part H2O2 6 in 2 10 [i.e., 500 parts per billion] using this method! Enzymatic methods

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33 Automating business processes Therefore, the functions of transport and connectivity to which we could add the function of information adaptation make it possible to handle the problems of application integration linked to the propagation of data At the same time, processing the cases that correspond to the classification type of multi-step process integration requires the complementary functions of business process automation This is the domain of integration of business processes, or BPM (Business Process Management) The WfMC (Workflow Management Coalition) defines a business process [WFM 99] as a collection of one or more linked procedures or activities that together accomplish a business objective, in the context of an organizational structure that define roles and relationships For example, in a financial institution, granting a loan is a business process which can be automated by chaining multiple applications, but that in certain cases could require approval from the service manager.

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The final type of replication goes by two different names. Under periodic, or batch-style, replication, data is saved up, and from time to time is replicated in a batch, all at once. Write ordering is not maintained, so during a replication cycle, the data that has reached the destination is not consistent. This means that if, during the replication cycle, the master system fails, the data on the destination system is not usable. The advantage to this style of replication is that it gives system and network administrators some control over when the network will be impacted by the additional load that replication introduces. Another important disadvantage to periodic replication is that the data on the destination system is very much out-of-date. If the data is updated via replication every 4 hours, for example, then on average, the data on the destination is 2 hours out-of-date. If an emergency causes a switchover from master to destination, as much as 4 hours of committed work can be lost. For most critical applications, this significant loss of data is considered unacceptable, and as a result, periodic replication is not considered acceptable for disaster recovery situations. Periodic replication is generally much more useful as a way of getting data to other systems for backups, data mining, and other noncritical uses.

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18

TABLE 2.1. Substrate Selectivity of Glucose Oxidasea Substrate b-D-Glucose 2-Deoxy-D-glucose 6-Deoxy-6- uoro-D-glucose 6-Methyl-D-glucose 4,6-Dimethyl-D-glucose D-Mannose D-Xylose a-D-Glucose Relative Rate of Oxidation (%) 100 25 3 1.85 1.22 0.98 0.98 0.22

If periodic replication is to be used for disaster recovery, then it is critical that additional copies of the data be maintained. The remote side will require a multisided data mirror, and while the replication is active, one side of the mirror must be broken off to receive the updates, while the other sides of the mirror hold their static (and consistent) copy of the data. Once the replication cycle has completed, the sides that were not updated will be reattached to the updated copy and will be brought up-to-date. Some implementations will require a similar process on the sending side, as a copy of that mirror is split off from the active copy, so that it does not change during the replication. Disk vendors love this method, as it allows them to sell multiple sets of disk spindles to keep up with all of the copies of the data set. Note that if it becomes necessary to recover from an older copy of the data (because of a failure during the copy), the data you ll get dates back to the previous round of replication.

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