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Since the developers might be kicked off their server at any moment, it means that they must change the way they work, saving their work far more often than they might otherwise If they forget to save frequently, then they could lose their work when a failover occurs It also gives the perception that their work is of limited value to the overall operation, since they may be asked to stop working at any time without warning In organizations where we have seen this model implemented, it has a detrimental effect on the morale of the developers and causes them to place less value on their own work and contributions to the organization In most cases, it just makes them angry.

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What s more, while critical services are running on their development server, the developers are unable to do any work, resulting in a slowdown in their performance, and delayed deliverables An additional cost to the business is the direct result of their idle time; they are collectively sitting around doing nothing, while collecting their wages and other benefits You re choosing one kind of downtime for another When evaluating the overall cost of.

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Figure 9.11. Comparison of the band separation of linear, wedge, and ionic strength gradient gels. The T reaction of a sequencing experiment carried out on M13 mp8 DNA was run on three different 40 cm 6% polyacrylamide gels, using (a) a standard linear gel, (b) a 0.35 1.05-mm wedge gel, and (c) a 0.05 0.50 M Tris buffer gradient. [Reprinted, with permission, from A. T. Bankier and B. G. Barrell, in Nucleic Acids Sequencing: A Practical Approach , C. J. Howe and E. S. Ward, Eds., Oxford University Press, New York, 1989. # IRL Press at Oxford University Press 1989.]

building a cluster in this manner, be sure to include the cost of idling developers and delayed deliverables.

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So, we ve decided that we shouldn t put our developers on the standby node in a two-node active-passive cluster. What does that leave What can we run on the standby node It is certainly okay to put another production-quality application on the standby node. Because of the nature of clustered systems, the applications must be totally independent of each other, using separate disks and separate networks. They should have no dependencies of any kind on each other. Within that constraint, it s just fine to put, for example, one instance of a database on one node and a second, separate, instance on the other node. Or a database instance on one node and a file-sharing service, such as NFS or CIFS, on the other. It s important to understand, though, that running any applications on the standby node increases the risk of that node not being available when it is required for a failover. A system that is busy running an application is measurably more likely to go down unexpectedly than is a node that is not running any applications. In our experience, though, for most applications and enterprises, the additional risk introduced by running truly well-tested and mature applications on the standby node is more than outweighed by the cost savings of actually using the standby node. When the alternative is to idle the standby system and buy two more hosts to cluster the other application, the cost savings are clear.

mixtures (the T reaction) using a 6% polyacrylamide gel in the linear, wedge and ionic strength gradient con gurations is shown in Figure 9.11. 9.5. DETECTION OF PROTEINS AND NUCLEIC ACIDS AFTER ELECTROPHORETIC SEPARATION Following an electrophoretic run, the band from the tracking dye is often the only visible band. The detection of separated proteins and nucleic acids requires subsequent treatment of the separation pattern for visualization. This treatment may be performed directly on the gel, or may require a blotting step in which the entire separation pattern is transferred onto a thin membrane material. The choice of detection method depends on the concentrations of analytes in the separated zones and whether recovery of the puri ed sample is required.

If maximum availability is the primary factor, and cost is a distant second, then the very best way to configure a pair of failover servers is to dedicate the standby server as a pure standby server, running absolutely no other applications on it. For many of the most critical applications, this configuration is fairly popular. But in other shops, where the costs of dedicating a server outweigh the availability benefits of putting one application in a cluster, this configuration turns out to be prohibitively expensive. However, by dedicating the standby server, you virtually guarantee its availability when it is needed. The rule is quite simple: The less the server does, the less risk there is that it will fail at an inopportune moment.

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