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The biggest issue when examining the issues of active-passive versus activeactive failover is, of course, the cost. Fundamentally, with active-passive you are buying two hosts to perform the work of one. One host sits largely idle

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cooling system In the absence of cooling, ve factors contribute to decreased resolution Convection currents occur because warmer solution near the center of the support has a lower density than cooler solution near the walls; water has a maximum density at 4 C, and at this temperature, density variation with temperature is minimal; runs should therefore be performed at 4 C where possible Diffusion, which leads to the blurring or widening of zones, is more signi cant at higher temperatures, especially if the run requires several hours A distortion of zones occurs when cooling is inadequate, since those parts of the zones in the warmer sections of the gel (the inside) move faster than those in the cooler (outside) portions; this unequal speed leads to curved bands, and sometimes to overlap with neighboring bands.

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most of the time, consuming electricity, administrative effort, data center space, cooling, and other limited and expensive resources. However, activepassive configurations are going to be the most highly available ones over time. Since there are no unnecessary processes running on the second host, there are fewer opportunities for an error to cause the system to fail. In general, largely due to the extra expense and administrative overhead, only the most critical applications run in an active-passive configuration. After an active-passive failover has completed (see Figure 17.3), the takeover server has control of the associated service group. Since both hosts in a shareddisk two-node cluster directly access the same disks (at different times), as long as the disks and all connectivity are in place, there is no question that the takeover host will see the same application data as the failed host.

In open vessels, evaporation may lead to surface dehydration of the support, and thus to local changes in ionic strength; for this reason covered vessels are used Finally, viscosity varies with temperature; higher temperatures lead to softer gels and lower frictional coef cients, an effect particularly noticeable with agarose gels The pH of the running buffer can have dramatic effects on the separation achieved This is not normally the case for nucleotides, polynucleotides, or nucleic acids with these species, the net charge is always negative due to the acidic sugarphosphate backbone, so that pH changes over the 4 10 range do not signi cantly affect separations For proteins, however, the net charge is pH dependent For pH values below the isoelectric point (pI), a protein will have a positive charge and will migrate toward the cathode.

Probably the most common question asked about active-passive failover configurations is How can I use the standby server It is most difficult to justify to the people who approve technology spending that they need to buy two identical servers, thus doubling the expenditure, while only actively using one of them at a time. So the obvious suggestion is to find some activity that can realistically and safely be performed on a standby node. This most commonly asked question is usually followed by this suggestion: What about my developers Can I put them on the standby server Of course, this is probably the worst thing to do with that standby server. The key question to ask when evaluating a potential activity for its fitness to be placed on the standby server is How likely is it that this activity will cause the server to be unable to accept a failover To correctly answer this question, you must consider the likelihood that whatever activity is taking place on your standby server will cause that server to crash, hang, freeze, or have its critical files altered. If the activity on the backup server causes a critical failover not to complete, extended downtime will almost certainly result. Another inevitable result will be you on the hot seat, trying to explain to your management why all the money that they spent to implement a highly available system did not do what it was designed to do. We have categorized many of the activities that a business might consider for placement on the standby server into four levels of advisability: Bad, Acceptable, Good, and Best.

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