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Cellulose acetate minimizes the adsorption effect, since most of the hydroxyl groups have been acetylated Separations using cellulose acetate are more rapid, with improved resolution and less tailing, leading to the facilitated detection of more concentrated spots or bands In addition, the background staining of cellulose acetate is lower than paper, since stain adsorption is also minimized Cellulose acetate has two other advantages over paper: it is transparent, facilitating optical detection of zones, and it dissolves easily in a variety of solvents, facilitating the elution and isolation of separated components 922 Starch Gels Starch was the rst gel medium used for an electrophoretic separation, and was introduced in 1958 The gel is prepared by heating a paste of potato starch in the electrophoresis buffer until the starch grains burst and the heterogeneous mixture becomes transparent and homogeneous.

Automated failovers are great to ensure that your systems recover quickly from most outages. But they are not enough. You must monitor your systems for any variety of error conditions, including system resource exhaustion (filesystems or memory fill-up), super-high CPU loads (which can resemble outages), network failures, and so forth. Most systems have automated monitoring tools that help trouble find your SAs, rather than the other way around. If the SAs don t have to spend time looking for problems, they are free to solve problems that find them, or to properly implement new systems. Framework products like BMC Patrol, Hewlett-Packard Open View, Tivoli Global Enterprise Manager, and Computer Associates Unicenter will monitor your systems for a variety of potential problems and then notify a preselected group of administrators via pagers, email, telephone calls, console displays, or other alarms. Generally these applications use a protocol called SNMP (Simple Network Management Protocol) to detect and pass on information about these errors. Make sure that your FMS and any homegrown or third-party applications are capable of sending SNMP notifications, so that any tool that subscribes to the pervasive SNMP standard is capable of monitoring it. This way, new applications can seamlessly merge themselves into the existing environment without requiring a new infrastructure. When selecting a system monitoring protocol or tool, as we said in our key design principles back in 5, be sure to select technologies that are mature and that have reference sites and web sites as sources of good information about successful deployments.

Every computer system needs to have some sort of console A console acts as a master terminal, often allowing access even when all other system resources are down Boot messages and other critical startup information appear on the console screen The problem, though, with fixed, attached consoles is that they make it very difficult to perform system maintenance, such as reboots, without being in the same room as the system If a system requires emergency work in the middle of the night (or any other time), fixed consoles require that your system administrators get in the car and visit the data center If your operating system supports text-based consoles, connect a terminal server as your console, rather than a fixed and local console GUI consoles, such as Windows operating systems generally require, are not practical for remote system management.

The hot solution is then poured into a horizontal tank and cooled to ambient temperature, yielding a semisolid gel As shown in Figure 97, a slot is cut into the gel, and sample is introduced as a slurry with starch grains The surface of the gel is then sealed with wax or grease Porous wicks are inserted directly into the ends of the gel, and function as electrical connections between the gel and the anodic and cathodic buffer compartments Following the electrophoretic run, the semisolid gel is removed from the tank, and is cut into layers for staining This preparative technique allows replicate layers to be stained with different reagents, and leaves macroscopic quantities of unstained.

Text-based consoles enable your system administrators to access systems from anywhere in the world, at any time of the day or night Few things are more frustrating to a system administrator than having to get up at 3:00 AM on a Sunday morning and drive to the office to repair a down server, only to discover that a single command she could have given to someone over the phone, or typed in herself, would have fixed the problem If she could have logged in from home, the system would have been back online faster, and she could have gone right back to sleep It s also wise if your system administrators have high-speed network access from their homes; such a program will probably pay for itself the first time that a system administrator is able to bring a system up from home without having to drive to the office.