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Sitting in front of the web server, load-balancing hardware and software may be used to distribute requests over multiple, identical web servers, and on the client side a proxy cache server sits between a client and server and caches commonly accessed pages so that requests don t have to go outside of the client s network Again, these systems can fail, making it appear that the web server has gone away Much like database servers, disks, filesystems, and logs can fill; memory or CPU can be exhausted; and other system resources can run out, causing hangs, crashes, unresponsiveness, and other nasty behavior How do your CGI programs react if they cannot write to a required file Or if they don t get a necessary response from some upstream application Make sure they continue to operate.

Usually a web server is a front end for an application server, that nexus of business logic, data manipulation routines, and interfaces to existing back-end systems that does the heavy lifting of a web site In the Java world, application.

See Ref. 15. [Reprinted, with permission, from P. G. Righetti, Isoelectric Focussing: Theory, Methodology and Applications , Elsevier, New York, 1983. # 1983, Elsevier Science Publishers B.V.]

In addition, to implement these rules, IT staff provide the set of technical elements required to translate the information defined by the business specialists. For example a business specialist describes the content of the purchase order, while IT staff construct its representation in XML (Figure 3.27). The business specialist describes the process of passing one event to another (Figure 3.25), and IT staff describe the characteristics of the adapters and the middleware that will carry out this process (Figure 3.28). To summarize, this interface should therefore be shareable between two populations, specialists in business and specialists in IT, and as a result, present the ergonomic characteristics and simplicity indispensable for use by non-IT staff. One of the solutions consists of dedicating distinct work environments that use technical and business transformation engines which share common objects such as descriptions of formats. It should moreover use an extremely powerful rules definition language in order to be able to deal with the complex cases of transformation and routing that we commonly find in information systems.

servers are the frameworks that run the J2EE environment. The overall reliability of the application server is a function of all of the components that are layered on top of it. If you re using J2EE, for example, but call a non-Java native method written in C or C++, that code poses a risk to the application server. Step on memory with some poorly written native code and it s possible to corrupt the memory footprint of the application server. Since web and application servers represent the top of the software stack, they re affected by the reliability and correct operation of all of the network pieces underneath them. Peter Deutsch, networking, security, and risk evaluation expert, points out that you can t control the security, reliability, or latency of a network. Furthermore, there s rarely a single point of control or single point of accountability for a network of systems. We ll look at how each piece of the networked system fits into the puzzle of making the overall system more reliable, based on the Availability Index that we ll introduce in 3.

17

The detection of enzymes by activity staining is important not only for the direct detection of separated enzymes, but also for the detection of noncatalytic proteins after staining with enzyme-labeled antibodies to the protein of interest The ability to detect a particular catalytic activity greatly reduces background staining, and allows the unequivocal identi cation of a particular analyte species in very complex sample mixtures 953 The Southern Blot16 This procedure was introduced by EM Southern in 1975, and is used to transfer DNA from agarose gels onto a nitrocellulose or nylon membrane for subsequent detection Transfer to nitrocellulose is accomplished after the gel has been soaked in a denaturing solution containing $ 1:5 M NaCl and 05 M NaOH; this disrupts the base pairing of double-stranded DNA, so that only the single-stranded form is present in the gel.

Client #1

The gel is then neutralized to a pH of $ 8, and placed in a transfer apparatus such as the one shown in Figure 914 Once the gel has been placed in the transfer apparatus, buffer in the pan is drawn through the gel by the wicking action of the lter paper The transfer proceeds over $12 h The upper lter papers are replaced when they are saturated with buffer The nitrocellulose membrane is then removed, air-dried and heated to 80 C for 2 h to ensure strong DNA binding Nylon membranes have largely replaced nitrocellulose for Southern transfers, because nylon has been shown to improve the transfer of small (< 200 base) DNA fragments, and it is more stable to pH extremes.

Client #2

Client #3